Figure 6.

N/C-Bid and Bax induce pore-like structures in the OG-LUV membrane. (A) Nontreated OG-LUVs seemed perfectly round and had smooth and continuous membranes. Vesicles were instantaneously frozen in vitrified ice and visualized by cryo-EM. (B) After a 5-min incubation with N/C-Bid and Bax, the liposome membranes exhibited regions of irregularity and some pore-like structures were visible (arrows). (C) After 60 min of incubation with N/C-Bid and Bax, almost all the vesicles exhibited membrane abnormalities (arrows). (D) In the presence of Bcl-xL, the presence of the pores and abnormal membranes were greatly diminished.