Table 1.

Cell lines used in this study

Cell name	Description	Reference
CCL39	Clonal hamster lung fibroblasts with endogenous NHE1	Pouyssegur et al. (1984)
PS120	NHE1-deficient cells derived from CCL39 fibroblasts	Pouyssegur et al. (1984)
WT	PS120 cells stably expressing wild-type NHE1	Denker et al. (2000)
KRA2	PS120 cells stably expressing NHE1-KR/A, which contains alanine substitutions for COOH-terminal juxtamembrane lysine and arginine residues and does not bind PI(4,5)P2 or ERM proteins but does retain H+ efflux	Denker et al. (2000)
E266I	PS120 cells stably expressing NHE1-E266I, which contains an E266I substitution and does not have H+ efflux but does retain PI(4,5)P2 and ERM binding	Denker et al. 2000
K2E	KRA2 cells stably co-expressing NHE1-E266I	This study
SN1	PS120 cells stably expressing the System N1 glutamine-H+ transporter	Chaudry et al., (1999)