works best either with high (300 mg) or very low (20 mg) amount of tissue, because of phenol-chloroform follows by ethanol precipitation steps and uses of denaturants and RNase inhibitors |
works best with low (<100 mg) amount of tissue, because filters may clog resulting in low yield of total RNA |
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Several chemical treatment steps in protocol may affect RNA quality |
Limited steps in protocol may not have influences on RNA intactness |
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Complex protocol, takes 4 hrs |
Simple protocol, takes only 20 min |
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chemically based aqueous-phase procedure, increased risk of cross contamination |
No phase separation step present, low risk of cross contamination |
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Use of hazardous chemicals like phenol and chloroform requires specific safety rules in the laboratory |
Absolutely safe while handling. |
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less expensive than SGC method |
More expensive than GTC method |