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Primer details: Sequence, annealing temperature (AT), and amplicon size (AS) of target sequence
| Target gene | Sequence (FP; 5' to 3') | AT [°C] | AS [bp] |
| Sequence (RP; 5' to 3') | |||
| GAPDH | AATGGTAAGGTCGGTGTGTGAA | 60 | 262 |
| GAAGATGGTGATGGGCTTCC | |||
| GAPDH-3' UTR | ACAGGGTGGTGGACCTCATG | 60 | 102 |
| GTTGGGATAGGGCCTCTCTTG | |||
| PBGD | TGCACGATCCTGAAACTCTG | 60 | 163 |
| TGCATGCTATCTGAGCCATC | |||
| 28S | TCATCAGACCCCAGAAAAGG | 60 | 102 |
| GATTCGGCAGGTGAGTTGTT | |||
| 18S | GCAATTATTCCCCATGAACG | 60 | 123 |
| GGCCTCACTAAACCATCCAA | |||
