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Type-I collagen enhances TNF-α induced activation of JNK by dermal fibroblasts. (A) HDFs were cultured on plastic or type-I collagen and stimulated by TNF-α for the indicted intervals. Phospho-JNK and JNK proteins were measured by Western blot analysis. (B) Activation of JNK was quantitated by densitometry analysis and plotted by the ratio of phosphor-JNK vs. JNK protein as arbitrary units.
