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Protein expression in BHK-21 cells transfected with replication-competent vectors containing inserted IRES elements. BHK-21 cells were transfected with equal amount of in vitro transcripts prepared from pSFV4-EMCV-d1EGFP, pSFV4-CR-d1EGFP, pSFV4-d1EGFP-MEMCV and pSFV4-d1EGFP-MCR, and cells transfected with transcripts from pSP6-SFV4 were used as controls. Analysis was carried out and materials are presented as described in Figure 2.
