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. 2009 Mar 24;6:33. doi: 10.1186/1743-422X-6-33

Figure 6.

Figure 6

Schematic presentation of the methods for replication-competent vector genome and library construction. (A) Construction of replication-competent vector from restriction fragments of pSFV-PLApa-d1EGFP (SphI to ApaI) and pHelper96 (ApaI to SpeI). SP6 corresponds to the promoter for SP6 RNA polymerase; bold arrow indicates the ligation process. (B) Construction of library from SphI/BamHI restriction fragment of pSFV4-T37/17 and BamHI-treated product of PCR-based mutagenesis, which contained 21-bp randomized fragment, coding sequence of d1EGFP and SFV4 3'-UTR with poly(A). SP6 corresponds to the promoter for SP6 RNA polymerase and bold arrow indicates the ligation process. Symbols have the same meaning as in Figure 1B. Only the ligation products corresponding to the cDNAs of replication-competent vector (A) or replication-competent vector-based library (B) are shown.