Table 1.
Comparison of genetic stability of different replication-competent SFV4-based vectors in BHK-21 cells
| P1 | P2 | P3 | P4 | P5 | |
| SFV4-T37/17-d1EGFP | 100% | 100% | 100% | 80% | 70% |
| SFV4-T26/20-d1EGFP | 100% | 96% | 85% | 59% | 41% |
| SFV4-T99/45-d1EGFP | 96% | 75% | 65% | 41% | 15% |
| SFV4-d1EGFP-M26 | 100% | 50% | 11% | 0% | 0% |
| SFV4-d1EGFP-M37 | 96% | 64% | 38% | 3% | 0% |
| SFV4-d1EGFP-M96 | 96% | 50% | 19% | 11% | 0% |
The d1EGFP expression was transient and relatively weak. Therefore, it is possible that some d1EGFP positive plaques were counted as d1EGFP negative and the stability of constructs was somewhat under-estimated.