Fig. 2.
The DNA encapsidation genes, ORFs 25, 26, 30, 34, 43, 45/42, and 54, were cloned into pcDNA3.1D/V5-His-TOPO. In vitro transcription and translation was used to synthesize C-terminal V5 epitope tagged polypeptides. Proteins were detected using an anti-V5 monoclonal antibody. The predicted molecular mass for each protein product with and without the V5 epitope is provided in Table I.