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Overview of the laboratory and analysis procedure. Tissue and blood samples were extracted using the EZ1 biorobot (Qiagen, step 2). Mt genomes were amplified as two overlapping fragments A and B (step 3), which were added to the respective pre-pipetted sequencing primers (step 4). Cycle sequencing products were analyzed by capillary electrophoresis (3100 Applied Biosystems, step 4).
