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PCR products of fragments A and B assessed in a polyacrylamide gel. (1) Amplification products of unpurified fragments A and B (each 5000 genomic equivalents). (2) Fragment A purified with filter plates (Microcon (Millipore), left side) and digestion (ExoSAP-IT USB, right side). PCR template amounts were 1000 (lane 1), 2500 (lane 2), 5000 (lane 3) and 10000 (lane 4) mtDNA genomic equivalents.
