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. 2009 Apr 24;4(4):e5331. doi: 10.1371/journal.pone.0005331

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Zhang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Protein samples (30 µg) of the total cell extract, membrane and soluble fractions of WT cells grown at LC were applied to SDS-PAGE and probed with Flv2, Flv3 and Flv4 antibodies. The antibodies against D1 and Rubisco large subunit were used to indicate the purity of the fractions. The corresponding Δflv mutants (Δ) served as negative controls. The experimental molecular weight of each Flv was estimated by comparing the migration of the respective proteins with the molecular weight markers in SDS-PAGE.