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. 2008 Nov;125(3):302–312. doi: 10.1111/j.1365-2567.2008.02857.x

Figure 5.

Figure 5

Regulatory T-cell analysis in mice deficient in the G-protein coupled receptor Gpr83. (a) Thymocytes were isolated from Gpr83−/− and age-matched wild-type (WT) BALB/c mice. The percentage of forkhead/winged helix transcription factor (Foxp3) and CD25 expression in CD4 single positive (SP) cells was determined by fluorescence-activated cell sorting (FACS). Results show the mean frequency of the indicated cell population in the total CD4 SP thymocytes (n= 3) ± standard deviation (SD). (b) Lymphocytes from WT and Gpr83−/− mice were isolated from the spleen (n= 12), mesenteric lymph nodes (MLN) (n= 12) and colonic lamina propria (LPL) (n= 4). The percentage of Foxp3 and CD25 expression in CD4+ T cells was determined by FACS. Results show the mean frequency of the indicated cell population in CD4+ T cells ± SD. (c) Individual spleens from WT and Gpr83-deficient mice were analysed for total cellularity, total CD4+ cell numbers and CD4+ frequency. Mann–Whitney statistical analysis was performed to determine the statistical significance of differences observed. KO, knockout.