Table 2.

Primers Used for Quantitative Real-Time PCR

Target gene	Accession mo.	Primers (5′-3′)	Position*	Product†
ALP	NM_000478	(F) ACCATTCCCACGTCTTCACATTTG	1131	162
		(R) AGACATTCTCTCTCGTTCACCGCC	1292
Runx2	NM_001024630	(F) GGACGAGGCAAGAGTTTCACC	777	51
		(R) GGGAGGATTTGTGAAGACGGT	827
GAPDH	NM_002046	(F) AGATCATCAGCAATGCCTCCTG	536	109
		(R) ATGGCATGGACTGTGGTCATG	644
ID1	NM_002165	(F) GGTGGAGATTCTCCAGCACG	283	51
		(R) TCCAACTGAAGGTCCCTGATG	333
MSX2	NM_002449	(F) CCACCCCCTCTAACGGCTAG	1144	50
		(R) AAATTTCAGCTATGTGGTGTGGC	1193
Osteocalcin	NM_199173	(F) ATGAGAGCCCTCACACTCCTC	1	293
		(R) GCCGTAGAAGCGCCGATAGGC	293
Osteopontin	NM_001040060	(F) TCCAACGAAAGCCATGACCA	149	359
		(R) TCCTCGCTTTCCATGTGTGA	508

* Position of primer relative to ATG start site.

^† Product size in base pairs.