Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 May 1;5(5):e1000408. doi: 10.1371/journal.ppat.1000408

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Asadulghani et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

(A) FIGE analysis of packaged phage DNA in untreated (−MMC) and MMC-treated (+MMC) cultures. Phage particles were collected from culture supernatants by PEG/NaCl precipitation. DNA preparations applied to the FIGE gel were obtained from 50-ml (untreated) and 1-ml (MMC-treated) cultures. (B) Quantification of packaged phage DNA in the culture supernatants obtained from untreated (−MMC) and MMC-treated (+MMC) O157 Sakai cells. DNase-resistant phage DNA was quantified by qPCR using the primers that were used in Figure 3. DNA preparations equivalent to the 1-ml culture supernatant were used as template DNA. The data were obtained from three independent analyses, and the average copy numbers for each prophage genome are shown. Bars indicate standard deviations. The Sp18 DNA and the chromosomal DNA from two chromosome regions (CB1 and CB2) were monitored as controls.