Table 2. Excision, replication, and packaging abilities of Sakai prophages and their transfer, lysogenization, and propagation in K-12.

Prophages	Circularization	Replication	Packaging	Transfer efficiencya				Lysogenization		Transfer from K-12 to K-12b
				MG1655		MC1061		MG1655	MC1061
				−MMC	+MMC	−MMC	+MMC
Sp1	−	−	−	N	N	N	N	N	N	N
Sp2	−	−	−	N	N	N	N	N	N	N
Sp3	−	−	−	N	N	N	N	N	N	N
Sp4	+	+	+	0	0	0	0	N	N	N
Sp5	+	+	+	6(4)	746(141)	2(1)	115(36)	+	+	+
Sp6	+	+	+	0	0	4(3)	0	N	+	−
Sp7	+	+	+	0	0	0	0	N	N	N
Sp8	−	−	−	N	N	N	N	N	N	N
Sp9	+	+	+	0	0	0	0	N	N	N
Sp10	+	+	+	0	0	47(16)	2	N	+	−
Sp11	−	−	−	N	N	N	N	N	N	N
Sp12	−	−	−	N	N	N	N	N	N	N
Sp13	+	+	+	0	0	0	0	N	N	N
Sp14	+	+	−	N	N	N	N	N	N	N
Sp15	+	+	+	0	8(5)	0	1	+	+	+
Sp16	−	−	−	N	N	N	N	N	N	N
Sp17	−	−	−	N	N	N	N	N	N	N
Sp18	N	+	+	N	N	N	N	N	N	N

^aTransfer to K-12 was examined using Cm^R–marked prophages. Average numbers of Cm^R colonies obtained in three or four independent experiments are shown. Standard deviations are given in parentheses. N; not applicable or not determined.

^bBoth K-12 strains MG1655 and MC1061 were used as recipients.