FIG. 2.

Wild-type cells formed buds next to the birth scar, whereas strains lacking Isw2p displayed bud scars within the birth scar. Cells were cultivated in liquid YPD medium overnight and double labeled with calcofluor white and WGA-FITC. The arrows indicate the birth scars in the wild-type (WTa; strain BY4741), isw2Δ MATa (isw2Δa; strain CRY3), and isw2Δ ste4Δ MATα (isw2Δste4Δα; strain CRY146) strains and chitin at the base of the shmoo-like projection in the isw2Δ MATα mutant (isw2Δα; strain CRY4). DIC, differential interference contrast. Bar, 5 μm.