FIG. 3.

The deletion of the DSE1 gene in the isw2Δ mutant (isw2Δa) background abolished the budding-within-the-birth-scar phenotype. In contrast to the isw2Δ cells (strain CRY3), the isw2Δ dse1Δ mutant (isw2Δdse1Δa; strain CRY671) displayed a common axial budding pattern, i.e., the bud was formed just outside of the birth scar. Cells were cultivated in the liquid YPD medium overnight and double labeled with calcofluor white and WGA-FITC. The arrow indicates the birth scar. DIC, differential interference contrast. Bar, 5 μm.