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Dse1-GFP was observed only in wild-type daughter cells. It formed a ring at the daughter side of the septum. Wild-type cells (strain CRY555) expressing GFP-tagged Dse1 from the chromosomal locus were cultivated in YPD medium overnight, then transferred to SC medium, and further cultivated under vigorous shaking for 3 h. Cells were stained with calcofluor white and analyzed for Dse1-GFP and calcofluor white fluorescence. DIC, differential interference contrast. Bar, 5 μm.
