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Overproduced Dse1-GFP localized to both sides of the septum forming a double ring in dividing cells (A), the signal persisted at the same sites during cell separation (B), and it was detected there even after the initiation of new budding (C to E). Cells carrying the pGAL-DSE1-GFP plasmid (strain CRY979) were cultivated overnight in SCRaff-Ura and then shifted to SCGal-Ura for 4 h. DIC, differential interference contrast. Bar, 5 μm.
