FIG. 6.

mRed-PpYap1 is transiently localized to the nucleus and positively regulates the transcription of genes related to the glutathione redox system during methanol metabolism. (A) Subcellular localization of mRed-PpYap1. The Ppyap1Δ mutant strain expressing mRed-PpYap1 was grown in glucose (SD) medium to exponential phase and shifted to methanol (SM) medium. (B) Nuclear accumulation of mRed-PpYap1 during growth on methanol. mRed-PpYap1-expressing cells incubated on methanol for 6 h were stained with DAPI. Bars, 2 μm. (C) Relative transcriptional levels of PpGLR1, PpGPX1, and PpGSH1 in the Ppyap1Δ mutant compared to those in the wild type. The relative abundance of these mRNAs was standardized against the levels for PpACT1. Results are means ± standard deviations (n = 3).