FIG. 7.

Defective gliding locomotion in siap-1(−) sporozoites. (A) Representative immunofluorescence pictures of sporozoites isolated from Anopheles midguts or hemocoels. Sporozoites were deposited onto coated coverslips, and trails were visualized with the anti-circumsporozoite protein (CSP) antibody. Note that siap-1(−) hemocoel sporozoites lack productive motility and are indistinguishable from midgut-associated sporozoites. (B) Quantification of the WT and siap1(−) motility patterns. Shown is the percentage of nonmotile, i.e., detached (white) and attached (light gray), sporozoites and sporozoites that display nonproductive (dark gray) and productive (black) motility. In contrast to WT sporozoites that mature upon egress to the mosquito hemocoel, siap-1(−) parasites remain nongliding.