TABLE 1.
In vivo infectivity of siap-1(−) and WT parasites
| Location of sporozoite population | Parasite population | Method of inoculation or no. of sporozoitesa | No. of infected animals/ inoculated animals | Prepatent period (days)b |
|---|---|---|---|---|
| Salivary gland | siap-1(−) | Mosquito bite | 0/4 | NA |
| WT | Mosquito bite | 8/8 | 3.5 | |
| siap-1(−) | 50,000 | 1/1 | 6 | |
| siap-1(−) | 10,000 | 0/5 | NA | |
| WT | 10,000 | 2/2 | 3.5 | |
| Hemocoel | siap-1(−) | 10,000 | 1/4 | 6 |
| siap-1(−) | 5,000 | 0/3 | NA | |
| WT | 1,000 | 10/10 | 4.7 | |
| Midgut | siap-1(−) | 1,000,000 | 1/1 | 5 |
| siap-1(−) | 400,000 | 1/2 | 7 | |
| siap-1(−) | 100,000 | 1/7 | 7 | |
| WT | 100,000 | 10/10 | 5.4 |
a
Young Sprague-Dawley rats were injected intravenously with indicated numbers of sporozoites or exposed to the bites of 5 to 10 infected mosquitoes at days 17 to 20 after mosquitoes were infected.
b
NA, not applicable.