FIG. 2.

Contribution of the interplay between TOF1 and RRM3 to plasmid integration and excision. (A) Autoradiogram of a representative Southern blot showing the effect of tof1Δ rrm3Δ double deletions on plasmid integration (pBB-Hyg) in comparison with the single deletions, all in a common sir2Δ background after three and six serial streakings and growth in the selective medium. (B) Effects of single and double deletions (tof1Δ, rrm3Δ, and tof1Δ rrm3Δ) on pBB3NTS (URA3) plasmid excision. (C) Autoradiograms of 2D gels showing replication fork arrest at Ter in different strains; the arrows show the termination spots generated by fork arrest at the two closely spaced Ter1 and Ter2 sites. WT, wild type.