FIG. 3.

Impact of deletions of genes involved in HR and NHEJ on plasmid integration and excision. (A) Autoradiogram of a representative Southern blot of nicked DNA showing the status of intracellular plasmid DNA pBB3NTS (URA3) in isogenic strains containing various deletions. The lanes are self-explanatory. (B) Plasmid excision kinetics showing the impact of various deletions of recombination genes on the excision of pBB3NTS (URA3). (C) Effect of deletions of two structure-specific endonucleases (slx4Δ, mus81Δ, and slx4Δ mus81Δ) on plasmid pBB3NTS (URA3) integration; the lanes are self-explanatory. WT, wild type.