TABLE 1.

Genes chosen for YFP tagging of Δku80

Toxodb gene IDa	Name	Mass (kDa)		Identified by:	No. of Toxodb MS peptidesb	Localization
		Predicted	Observed (with YFP)			Expected	Observed
55.m00019	ACP	19	50, 43	Waller et al. (46)	11	Apicoplast	Apicoplast
50.m00004	pCNA	35	60	Guerini et al. (14)	8	Nucleus	Nucleus
583.m00003	ROP1	48	75	Ossorio et al. (28a)	24	Rhoptries	Rhoptries
641.m00002	MIC3	38	NAf	Zhou et al. (49)	53	Micronemes	Micronemes
20.m03849	PLP1	113	160	Zhou et al. (49)	16	Micronemes	Micronemes
65.m00002	MIC5	26	NA	Zhou et al. (49)	21	Micronemes	Subapical
33.m00006	M2AP	35	NA	Zhou et al. (49)	72	Micronemes	Subapical
50.m00006	HSP60	61	NA	Toursel et al. (41a)	>100	Mitochondria	No signal
44.m00004	IMC1	70	NA	Mann et al. (23)	>100	IMC	No signal
44.m00009	P30/SAG1	30	NA	Kasper et al. (18a)	35	Surface	Anterior to nucleus
645.m00037	Cathepsin protease L	47	70	Putative MIC maturase	2	MVEc/lysosome	Anterior to nucleus puncta
83.m01237	Chitinase-like	72	125	Zhou et al. (49)	11	Micronemes	Micronemes
83.m00006	SPATR	58	95	Kawase et al. (19)	3	Apical	Micronemes
20.m03858	Hypothetical	97	130	Invasion cell cycle	9	Unknown	Micronemes
20.m03958	Toxolysin 4	256	NA	Zhou et al. (49)	26	Unknown	Subapical
49.m00054	Hypothetical (dystroglycan dom.)	101	140, 100	Zhou et al. (49)	6	Unknown	Subapical
55.m04865	Hypothetical				3	Unknown	Subapical
49.m03355	Hypothetical	12	40	Invasion cell cycle	14	Unknown	Apical tip/conoid
72.m00001	Non-TM Ag [poly(ADP-ribose) glycohydrolase]	62	85	Invasion cell cycle	49	Unknown	PVg
20.m03748	C2 domain protein	33	55	Invasion cell cycle	9	Unknown	Cytosolic
50.m00008	Cathepsin protease B	62	NA	Que et al. (30a)	5	Rhoptries	No signal
55.m04618	Hypothetical (25 EGFd)	172	NA	MIC-like domains	1	Unknown	No signal
8.m00176/8h	Hypothetical (4-PANe)	39	NA	Zhou et al. (49)	2/12	Unknown	No signal
80.m02161	Hypothetical	48	NA	Invasion cell cycle	>100	Unknown	No signal

^aAs of October 2008.

^bPeptide(s) identified by mass spectroscopy according to Toxodb.

^cMVE, multivesicular endosome.

^dEGF, epidermal growth factor.

^eAlso known as Apple domains.

^fNA, not applicable.

^gPV, parasitophorous vacuole.

^hGenes 8.m00176 and 8.m00178.