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Phenotypes of the strains used in this study. (A) Growth of wild-type (wt) and mutant strains on CM plates in the absence (top row) or in the presence (bottom row) of 10 μg/ml tunicamycin (tm). (B) Microscope images of the wild-type strain, the hacA deletion strain (ΔhacA), the strain bearing the intronless hacA gene (Δintron), and the strain bearing the unspliceable hacA variant (mut-intron).
