TABLE 1.
PCR oligonucleotide primers used in this studya
| Oligonucleotide | Sequence | Description |
|---|---|---|
| P1 | 5′-GGAGAACCAAGGTGCTATTG | −2,071-bp hacA start codon |
| P2 | 5′-GTTTAGGAGCCTTTACCTCC | +1,152-bp hacA stop codon |
| P3 | 5′-ATTTACCGTACGGTCAATTGGGGC | −503-bp hacA start codon |
| P4 | 5′-CCCTGCCTGTACTGACGAGTCATC | +120-bp hacA start codon |
| P5 | 5′-GGCCTGATCTGAACACGGACGCCTTTTAAAGAGT | UPRE1 |
| P6 | 5′-TCCCCCGTTATGACACGGACGCCTGTGTTCCTGT | UPRE2 |
| P7 | 5′-ATGGTTCTTAAGGACACCACTCCTTCTTGGCCCT | UPRE3 |
| P8 | 5′-TCTTTTTATTGTTCTCTGGTTCTTAAGGACACC | ATG uORF |
| P9 | 5′-CGACCTACATCACCGTCCTCCCAACGTCAGCGGTTAAGATAAGGCTCATAGTAAATCGATTG | 5′ splice site |
| P10 | 5′-TAGTGCCGTCCTCTGCGATCTTCA | Unspliceable intron us |
| P11 | 5′-TGAAGATCGCAGAGGACGGCACTA | Unspliceable intron ls |
| P12 | 5′-GCCTGGGTTAGCGCCCCCTGCAAGCCCCGTTATGACACGGTGGCCTGTGTTC | Inverted repeat 5′UTR |
| P13 | 5′-GACAGGTAATTCCTGCCCCCATGACTTTCTCTTCTTCACAGG | Inverted repeat hacA gene |
| P14 | 5′-TCCCCCGTTATGACACGGTGGCCTGTGTTCCTGT | −168-bp hacA start codon |
| P15 | 5′-TCAAACCGCTCAAGATTCGTTT | +1,253-bp hacA start codon |
| P16 | 5′-CGTCGAGAACGTCAAAGGCGAACCCGTC | For 5′RACE hacA |
| P17 | 5′-TTCGATGACAAGGATGTCCAGA | For RT-PCR bipA |
| P18 | 5′-GGGGATGAGCTTGGTCATGA | For RT-PCR bipA |
| P19 | 5′-ATTTAAATACCCTCTCCCATCGTCCTC | For RT-PCR pdiA |
| P20 | 5′-CCTCCTCGGCGGTGCAGTCAACCTTCAC | For RT-PCR pdiA |
a
Mutations are represented in boldface type.