TABLE 2.

Frequency of RNAP II::GFP dispersal in the wild-type, cdk1F, Anfcp1^TS, and Anfcp1^TS cdk1F strains at 37°C^a

Expt	No. of cells (%)
	Wild type		cdk1F		Anfcp1ts		Anfcp1ts cdk1F
	Dispersed	Total	Dispersed	Total	Dispersed	Total	Dispersed	Total
1	8	234	9	220	43	726	76	797
2	19	515	22	530	46	571	46	543
3	31	812	31	826	30	851	81	822
Total	58 (3.7)	1561	62 (3.9)	1,576	119 (5.5)	2,148	203 (9.4)	2,162

^aCells of individual strains were grown for 8 h at 37°C and fixed. The cells in which the RNAP II::GFP signal was dispersed were counted, as well as the total number of cells. The experiments were repeated three times independently. Student t test analysis indicated that only the difference between the wild-type and the Anfcp1^ts cdk1F strains was significant (P < 0.0005).