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Cla4 localizes with the vacuole. (A) Cla4-GFP was visualized in asynchronous wild-type (YCH3250) cells. Arrows indicate perivacuolar Cla4-GFP localization, and arrowheads indicate cortex localization. (B) CLA4-GFP (YCH3250) cells were FM 4-64 stained and examined by fluorescence microscopy.
