FIG. 3.
Cells lacking PAK function form but do not resolve segregation structures. (A) Mid-logarithmic-phase FM 4-64-strained wild-type (W303-1A), cla4 (KN3591), and ste20 (KN3621) strains were examined by fluorescence microscopy. (B to D) Asynchronous FM 4-64-stained wild-type (W303-1A) and cla4-75 ste20 (KN4580) cells were elutriated to obtain a uniform population of G1 cells, grown at 37°C, and examined at the indicated times by fluorescence microscopy. Values in panels B and C are the percentages of cells with a daughter vacuole.