FIG. 7.
LTE1 is required for degradation of Vac17 in CLA4-overexpressing cells. (A) Mid-logarithmic-phase lte1 (YCH4737) and GAL-CLA4 lte1 VAC17-ProA (YCH4934) cells were grown in YP plus 2% raffinose and FM 4-64 stained. At the zero time point 2% galactose was added to induce CLA4 overexpression, samples were taken every hour, and Western blot analysis was performed and vacuole inheritance was quantified. (B) Mid-logarithmic-phase GAL-BUB2 bub2 lte1 ste20 Vac17-ProA (YCH5286) cells were grown in YP plus 2% raffinose, 2% glucose was added at time zero, and samples were taken at the indicated times and subjected to Western blot analysis. Additionally, the percentage of telophase cells was determined by DAPI staining and scoring the number of cells with mother and bud nuclei staining.