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Primers used for PCR cloning and RT-PCR
| Primer | Nucleotide sequence (5′-3′)a |
|---|---|
| CpSUB1-F | ATGAAAAAGGTAAATATTTTCAAGTTGTTG |
| CpSUB1-R | TCATGAATCTAACCTACCAAATTCATT |
| CpSUB1-CDF | AAAGGATCTGGAGTATATTAT |
| CpSUB1-CDR | AATATTTAATGTCCCAGAGG |
| CpSUB1-PDF | GGTATTGAGGGTCGCAGATCTAAAGTTATTCCAGGA |
| CpSUB1-PDR | AGAGGAGAGTTAGAGCCATATTCAACTTCATCACTATG |
Underlined nucleotides represent overhangs required for ligation-independent cloning into the pET 32Xa/LIC vector.
