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. 2009 Apr 24;4(4):e5315. doi: 10.1371/journal.pone.0005315

Figure 2. 8-azido-ATP binding to SVOP-FLAG is saturable and displays a binding affinity of 83 µM.

Figure 2

Purified SVOP-FLAG was labeled with the indicated concentrations of 8-azido-ATP-biotin and subjected to SDS-PAGE and western blot analysis. The net intensity of the regions of interest was quantified using a Kodak Image Station 440. A, Representative western blot result of SVOP-FLAG labeling as a function of 8-azido-ATP concentration. B, Quantification of the western data. The data were expressed as the intensity of 8-azido-ATP labeling normalized to SVOP protein signals. The error bars represent SEM (n = 4).