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. 2009 Apr 23;4(4):e5305. doi: 10.1371/journal.pone.0005305

Figure 3. Blocking VGCC increases calcium release from intracellular stores.

Figure 3

Real time increase in calcium influx over 5 min in CFP10-DCs stimulated with 1 MOI BCG. Prior to stimulation, DCs were blocked with anti-L-type antibody (b, e & h) or anti-R-type antibody (c, f, & i). Subpanels a, d and g represent BCG stimulation in the absence of VGCC blocking. For panels A and B, DCs were suspended in calcium sufficient (RPMI) medium and calcium (PBS) deficient medium, respectively. For panel C, DCs were incubated with 100 µM TMB-8 prior to blocking with L-type or R-type VGCC and calcium measurements were carried out in calcium sufficient (RPMI) medium. Data from one of three independent experiments are shown.