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. 2009 Apr 23;4(4):e5273. doi: 10.1371/journal.pone.0005273

Figure 5. P140-induced changes in expression of inflammation/tolerance/anergy-related genes in MRL/lpr mice.

Figure 5

T cell anergy and tolerance RT-PCR-Array were used to evaluate expression of a selected set of genes expressed by non-activated T CD4+ cells from 6 week-old CBA/J mice, MRL/lpr mice and P140-treated MRL/lpr mice. (A) Heat map of differentially-expressed genes between 6-week-old normal CBA/J and MRL/lpr mice and between non-treated MRL/lpr and MRL/lpr mice that received each a single intravenous injection of 100 µg P140 peptide (evaluated 6 h and 24 h post-administration). A scale of colour intensity is presented for each heat diagram. Red, up-regulation; green, down-regulation; black, no change. Genes included in the microarray are listed in the table. Results are the average in gene expression for four independent experiments, with three mice per group in each experiment. (B) Changes in gene expression between non-activated T CD4+ cells from CBA/J and non-treated MRL/lpr. Out of the 84 genes tested, four were significantly up-regulated and one significantly down-regulated. The other changes were not statistically-significant. (C) Changes in gene expression between non-activated T CD4+ cells purified from non-treated MRL/lpr mice and MRL/lpr mice collected 6 and 24h post-treatment. (D) PBL surface expression of PD-1 studied in the three groups of mice by flow cytometry. Mean fluorescence intensity (MFI) values are indicated.