Figure 1.
Inhibition by peptides 29 (Panel A) and 30 (Panel B) of binding of Shc to hepatocyte growth factor receptor (c-Met) tyrosine kinase in intact B5/589 cells as described in Experimental Section. Cells were incubated for 1 h in presence (10 μM) or absence (0 μM) of the indicated peptides, then untreated (control) or briefly stimulated with HGF (+HGF). Cells were lysed and the amount of Shc bound to c-Met determined (upper panels) as well as the amount c-Met recovered (lower panels).