Skip to main content
. Author manuscript; available in PMC: 2010 Mar 26.
Published in final edited form as: J Med Chem. 2009 Mar 26;52(6):1612–1618. doi: 10.1021/jm800789h

Table 3.

Shc SH2 domain-binding affinities of bis N-olefin containing peptides.

graphic file with name nihms-96662-t0014.jpg
Peptide A Analogues Peptide B Analogues
No. Positiona,b n IC50 ± s.d. (μM)c No. Positiona,b n IC50 ± s.d. (μM)c
31 0 1 189 ± 15 38 0 1 91 ± 12
32 1 1 353 ± 34 39 1 1 81 ± 6
33 2 1 48 ± 2 40 2 1 47 ± 2
34 3 1 41 ± 7 41 3 1 450 ± 38
35 0 2 165 ± 4 42 0 2 84 ± 4
36 1 2 89 ± 5 43 1 2 84 ± 6
37 2 2 8 ± 2 44 2 2 14 ± 3
a

Site of N-allyl substitution.

b

A designation of “0” indicates the lack of N-allyl substitution.

c

Determined by a fluorescence anisotropy based competition assay using peptide 8 as a reference as described in the experimental procedures.