Table 4.
Shc SH2 domain-binding affinities N-alkylglycine containing peptides.
 | ||||||||
|---|---|---|---|---|---|---|---|---|
| No | R | IC50 ± s.d (μM)a | No | R | IC50 ± s.d (μM)a | No. | R | IC50 ± s.d (μM)a |
| 45 |  |
248 ± 5 | 52 |  |
83 ± 3 | 59 |  |
16 ± 4 |
| 46 |  |
19 ± 0.5 | 53 |  |
17 ± 3 | 60 |  |
17 ± 3 |
| 47 |  |
25.7 ± 0.7 | 54 |  |
40 ± 6 | 61 |  |
33 ± 1 |
| 48 |  |
26.6 ± 0.7 | 55 |  |
23 ± 3 | 62 |  |
34 ± 1 |
| 49 |  |
8.9 ± 0.1 | 56 |  |
56 ± 13 | 63 |  |
20 ± 3 |
| 50 |  |
6.0 ± 0.2 | 57 |  |
29 ± 7 | 64 |  |
14 ± 0 |
| 51 |  |
18.9 ± 0.3 | 58 |  |
10 ± 0.7 | 65 |  |
44 ± 2 |
a
Determined by a fluorescence anisotropy based competition assay using peptide 8 as the reference peptide as described in the experimental procedures.