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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2008 Nov 21;50(3):1392–1399. doi: 10.1167/iovs.08-2868

Figure 1.

Figure 1

Production of the C3 cleavage product iC3b in response to A2E photooxidation. The effect of depletion of complement components factor B and C1q. (A) Levels of iC3b are reduced when factor B is depleted. iC3b levels in NHS or factor B-depleted serum overlying RPE cells that had or had not accumulated A2E and were irradiated or not irradiated to generate A2E photooxidation products. (B) iC3b content is elevated in C1q-depleted serum placed in contact with RPE cells that had accumulated A2E and were irradiated (A2E 430 nm) to generate A2E photooxidation products. iC3b was measured by enzyme immunoassay. A suspension of zymosan in serum served as positive control. Values measured in normal and depleted serum incubated in otherwise empty wells were subtracted as background. Mean ± SEM; three or four experiments, duplicate wells per experiment.+, presence of condition.