Fig. 6.

Phonotaxis to overlapping sources with large onset delays (100 ms, 200 ms, and 500 ms). Two synthetic sound sources broadcast with 100 ms onset delay (half-overlapping pulse train), 200 ms onset delay (continuous pulse train), and 500 ms onset delay (2 distinct chirps separated by 300 ms of silence) (Insets, A and B). (A) Average walking responses of flies (n = 7 flies) to sources spatially separated by 90° with onset delays of 100 ms (black), 200 ms (blue), and 500 ms (red). (B) Average walking responses (n = 7 flies) of flies to sources spatially separated by 180° with onset delays of 100 ms (black), 200 ms (blue), and 500 ms (red). Square symbols and horizontal/vertical projections from symbols indicate the mean and confidence intervals of the location of flies at 0.07-ms (A) and 0.03-s (B) intervals. Filled symbols indicate walking responses to forward (A) or left (B) leading sources while open symbols indicate walking response to lateral (A) or right (B) leading sources. Under all temporal overlaps tested, flies initially turned and walked toward the leading source followed by a secondary turning response to the lagging source. Secondary turning responses were weaker for sources separated by 180°.