Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Mar 30;106(15):6339–6344. doi: 10.1073/pnas.0811741106

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

PMC Copyright notice

Fig. 1. — QS phenotype diversity of isolates from intubated patients. (A) Elastase and rhamnolipid production of all 364 isolates was measured by ECR assay and halo diameter on modified SW-blue plates, respectively, and scored with respect to PAO1 activity (set to 100%), as shown in B. Genotypes of isolates from each individual patient were compared by RAPD. (B) The lasR and rhlR genes were sequenced in at least one isolate of each QS class from every patient. The presence of mutated lasR and rhlR alleles in the remaining isolates was assessed by high resolution melting (HRM) analysis of the corresponding PCR amplicons. Isolates of this class may carry either a wild-type (wt) or a mutated (mut) allele of the gene. (C) Diversity (H) of colonizing isolates across the 31 patients as calculated by the Shannon–Weaver index (19).