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. 2009 Apr 6;106(15):6327–6332. doi: 10.1073/pnas.0813199106

Fig. 4.

Fig. 4.

Role of phosphate on the activation of pyoverdin system in P. aeruginosa PAO1. (A) Effect of exogenous PQS, 40 μM on pyoverdin production by P. aeruginosa PAO1 grown in NGM↓Pi and NGM↑Pi liquid media. (B) Production of pyoverdin in PAO1 and its derivative mutant ΔPqsAH grown in NGM↓Pi liquid media. (C) Dose dependent effect of Fe2(SO4)3 on pyoverdin production at varying concentrations of K-Ph buffer added to NGM↓Pi while keeping pH constant at 6.0 with 25 mM Mes buffer. (D) Expression of phosphate (pstS) and iron (pvdA, fpvA, pvdS, pchF) related genes in P. aeruginosa PAO1 grown in NGM↓Pi and NGM↑Pi liquid media with or without supplementation of 10 μM Fe3+.