Fig. 2.

Notch1 restores tumor growth in the absence of β-catenin/TCF signaling. (A and B) A total of 1.5 × 10⁶ cells were implanted s.c. in nude mice [Ls174T/dnTCF4 (black arrow) and Ls174T/dnTCF4/N1IC (red arrow) clones] untreated or treated with doxycycline for 4 weeks (2 independent experiments, n = 20 mice each). Representative animals for each group (A) and average and SEM of the tumor volume (in cubic millimeters) (B) are shown. P values are based on a nonparametric ANOVA applying a rank transformation on the dependent variable. (C) Alcian blue staining and IHC with α-Ki67 in serial sections of representative tumors. α-flag or α-myc staining shows the expression of dnTCF4 and N1IC (400×).