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. 2009 Mar 26;106(15):6315–6320. doi: 10.1073/pnas.0813221106

Fig. 5.

Fig. 5.

Notch1 and Notch2 are activated in colorectal tumors. (A) Levels of Jagged1 mRNA as measured by qRT-PCR. The red line indicates the average value for each group. (B) Serial sections of colorectal adenoma from a FAP patient stained with the indicated antibodies. Dashed lines indicate the boundary between the normal adjacent (N) and the tumor (T) tissue. Hematoxylin (blue) was used for nuclear staining. (C) Immunoflouresence with α-N1ICv (green) of normal or adenoma tissue from FAP sample. Nuclei were stained with DAPI (blue) (600×). (D) qRT-PCR to determine the levels of the indicated genes in normal tissue compared with FAP samples. The red line indicates the average value for each group. Statistical significance of the differences between the adenoma and normal samples are calculated based on 2-sided Student's t test. P values are: EphB3, P = 0.014; CD44, P = 0.0002; SOX9, P = 0.005; NOX1, P = 0.002; KLF5, P = 0.01; and hes1, not significant.