Fig. 1.

Melanoma identification and laser capture microdissection of melanoma cells. (A) A pigmented lesion on the left upper arm of a 52-year-old XP patient, XP295BE, showing the melanoma features of asymmetry, border irregularity, color variation, and large diameter. (B) Dermatoscopic image of A (20× magnified) showing a central, asymmetric, homogeneously hyperpigmented area with irregularly distributed black dots (arrows) and irregular streaks (arrowheads). (C) Atypical melanocytes are present singly and in nests at the dermal-epidermal junction (arrows) and show focal extension into the superficial dermis (hematoxyalin and eosin stain). (D) Melan-A staining showing melanoma cells expressing Melan-A (arrows). (E) Tissue remaining after capture of the melanoma cells showing high efficiency of capture (arrows). (F) Nests of melanoma cells captured (arrows) along with small amount of adjacent tissue.