Fig. 3.

Real-time self-assembly of LMM shows MSM mutants are assembly defective. The amount of 90° light scattering for no salt buffer (10 mM TES, 3.5 mM EDTA, 1 mM TCEP) was observed for 120 s before the addition of protein to obtain a baseline for scattering. An equal volume of either WT (black) or mutant (gray) LMM in high salt buffer was then injected into the sample chamber, diluting the sample to a final concentration of 150 mM NaCl and 100 nM protein to initialize self-assembly. Reactions were followed for 40 min before the addition of 5M salt to return the sample to a final salt concentration of 300 mM to demonstrate the reversibility of the reaction. The intensity of the 90° light scattering is plotted in arbitrary units versus time.