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R1845W and H1901L LMM form drastically larger paracrystals. Paracrystals were formed by diluting the protein from high salt buffer to low salt buffer, similarly to static light scattering experiments, but to a final protein concentration of 200 nM. Reactions were then allowed to proceed for 45 min at room temperature to allow for complete assembly before molecular sizing experiments were performed. Native protein denotes WT LMM protein, which was measured before assembly as a control.
