Fig. 1.

iNKT cells negatively regulate IL-17 production by CD4⁺ T cells. Purified CD4⁺ T cells were stimulated for 72 h with plate-bound anti-CD3 and anti-CD28 mAbs and IL-23. (A) Release of IL-17 (Left), IL-4 (Middle), and IFN-γ (Right) by CD4⁺ T cells from (A) wild-type (WT) or CD1d^−/− C57BL/6 mice or (B) wild-type (WT) or Jα18^−/− C57BL/6 mice was assayed by ELISA. Each data point represents the mean ± SEM of 2 (A) or 3 (B) experiments.