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. 2009 Mar 23;106(15):6375–6380. doi: 10.1073/pnas.0901515106

Fig. 4.

Fig. 4.

Effect of FABP inhibition upon AEA internalization and hydrolysis by FAAH. (A) Treatment of COS-7-FAAH-eGFP and N18TG2 cells for 5 minutes with 100 μM oleic acid, a ligand for FABPs, significantly reduced [14C]AEA uptake (black bars) and hydrolysis (gray bars). *, P < 0.05 compared with vehicle-treated controls (n = 3–5). (B, C) Treatment with 20–100 μM BMS309403, a selective competitive FABP inhibitor, significantly decreased [14C]AEA uptake and metabolism by COS-7-FAAH-eGFP (B) or N18TG2 (c) cells at 5 min. *, P < 0.05 and **, P < 0.01 compared with vehicle controls (n = 3–5). (D, E) Increasing concentrations (20–100 μM) of BMS309403 reduced the hydrolysis of [14C]AEA after cellular uptake by COS7-FAAH-eGFP (D) or N18TG2 (E) cells at 3 sec. *, P < 0.05 and **, P < 0.01 compared with vehicle-treated controls (n = 3–5).